MTT
Update:2014/11/3 6:24:10
| Item: | MTT | ||
| Synonym: | Thiazoyl Blue Tetrazolium Bromide; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide; Methylthiazoletetrazolium | ||
| CAS No.: | 2478-38-8 | Special Grade: | Ultra Pure Grade |
| Formula: | C18H16N5SBr | M.W.: | 414.3 |
Order
| Product No. | Size | Price | Brand |
| 0793-1g | 1g | 210 | GBCBIO |
| 0793-5g | 5g | 960 | GBCBIO |
| 0793-25g | 25g | 3750 | GBCBIO |
| 0793-5g | 5g | 1433 | Amresco |
Introduction
| Purity (%)..............................................≥98.0 Melting Range(°C)..................................≥187 |
| Demonstrates oxidative systems by formation of a metal-formazan chelate precipitate. MTT主要用來檢測細(xì)胞存活和生長狀況。其檢測原理為活細(xì)胞線粒體中的琥珀酸脫氫酶能使外源性MTT還原為水不溶性的藍(lán)紫色結(jié)晶甲瓚（Formazan）并沉積在細(xì)胞中，而死細(xì)胞無此功能。二甲基亞砜（DMSO）能溶解細(xì)胞中的甲瓚，用酶聯(lián)免疫檢測儀在490nm波長處測定其光吸收值，可間接反映活細(xì)胞數(shù)量。在一定細(xì)胞數(shù)范圍內(nèi)，MTT結(jié)晶形成的量與細(xì) |
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